yisJ [2019-06-27 12:39:34]

unknown

locus: BSU10740

pI: 8.05

mw: 35.53 kDa

protein length: 307 aa

gene length: 924 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

Gene

Coordinates: 1,151,166 1,152,089

The protein

Protein family

• cotH family (according to Swiss-Prot)

Structure

• [PDB|5JD9] (from B. cereus, 28% identity)

[SW|Localization]

• spore wall (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|75FBA935A39AF67DB4E69C8699D29D9F5C149143|yisJ]-[gene|022933D89666CDD2ACE39BCBB78D349BAF8E899B|yisI]
  description: [Pubmed|22383849]

  regulation

  • induced during [SW|sporulation] [Pubmed|22383849]
  • strongly expressed during oligotrophic growth [pubmed|30792386]

Biological materials

Mutant

• MGNA-B189 (yisJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1188 NBRP B. subtilis, Japan]
• BKE10740 ([gene|75FBA935A39AF67DB4E69C8699D29D9F5C149143|yisJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCTTTCCTCCTTCTC, downstream forward: _UP4_TAGTCCTTTCTCCATTAAAA
• BKK10740 ([gene|75FBA935A39AF67DB4E69C8699D29D9F5C149143|yisJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCTTTCCTCCTTCTC, downstream forward: _UP4_TAGTCCTTTCTCCATTAAAA

References

Research papers

• 30792386